human ( Homo sapiens )
eIF2C4

Western result: +

GST GST-eIF2C4 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: eIF2C4
CBI Antibody Core Code: A1178
"Lot" Number: 1 Position: 100 (587-686)
% Identical Homology of Target to Mouse Protein: 95% (NP_694817.1| Piwi/Argonaute family protein meIF2C4; argonaute 4)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
GADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTS
RQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFKPTRI
IYYRGGVSEGQMKQVAWPEL

Sequence Name: eIF2C4
Full name:
UniGene Number: N.A.
Accession Number: NM_017629
Source organism: human ( Homo sapiens )
Full length size (aa): 861
NCBI information page:

Full length sequence:
MEALGPGPPASLFQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVD
IKPEKRPRRVNREVVDTMVRHFKMQIFGDRQPGYDGKRNMYTAHPLPIGR
DRVDMEVTLPGEGKDQTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQ
ALDVITRHLPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAM
WNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTK
EIRGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQ
YFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQ
TSTMIKATARSAPDRQEEISRLVKSNSMVGGPDPYLKEFGIVVHNEMTEL
TGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQFYAGIEIKVWAVACFAP
QKQCREDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHL
KMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQ
TLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKK
PSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRE
LLIQFYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDY
RPGITYIVVQKRHHTRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDF
YLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVS
IPAPAYYARLVAFRARYHLVDKDHDSAEGSHVSGQSNGRDPQALAKAVQI
HHDTQHTMYFA