human ( Homo sapiens )
PIP5KIgamma

Western result: +

control PIP5KIgamma A1155b (fragment)

33.08 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: PIP5KIgamma
CBI Antibody Core Code: A1155b
"Lot" Number: 1 Position: 28 (641-668)
% Identical Homology of Target to Mouse Protein: 89% (NP_032870.1| phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma)
Molecular Weight of GST-fusion: 33.08 KDa

Target sequence used to make this antibody:
PTDERSWVYSPLHYSAQAPPASDGESDT

Antibody Name/Abbreviation: PIP5KIgamma
CBI Antibody Core Code: A1155a
"Lot" Number: 1 Position: 20 (549-568)
% Identical Homology of Target to Mouse Protein: 95% (NP_032870.1| phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma)
Molecular Weight of fusion protein: 32.2 KDa

Target sequence used to make this antibody:
YRRRTQSSGQDGRPQEEPPA

Sequence Name: PIP5KIgamma
Full name: phosphatidylinositol 4 phosphate 5 kinase I gamma
UniGene Number: N.A.
Accession Number: NP036530
Source organism: human ( Homo sapiens )
Full length size (aa): 668
NCBI information page:

Full length sequence:
melevpdeaesaeagavpseaawaaesgaaaglaqkkaaptevlsmtaqp
gpghgkklghrgvdasgettykkttsstlkgaiqlgigytvghlsskper
dvlmqdfyvvesiffpsegsnltpahhfqdfrfktyapvafryfrelfgi
rpddylyslcneplielsnpgasgslfyvtsddefiiktvmhkeaeflqk
llpgyymnlnqnprtllpkfyglycvqsggknirvvvmnnilprvvkmhl
kfdlkgstykrraskkekeksfptykdldfmqdmpegllldadtfsalvk
tlqrdclvlesfkimdyslllgvhnidqhererqaqgaqstsdekrpvgq
kalystamesiqggaargeaiesddtmggipavngrgerlllhigiidil
qsyrfikklehtwkalvhdgdtvsvhrpsfyaerffkfmsntvfrknssl
ksspskkgrggallavkplgptaafsasqipsereeaqydlrgarsyptl
edegrpdllpctppsfeeattasiattlsstslsiperspsetseqpryr
rrtqssgqdgrpqeeppaeedlqqitvqvepacsveivvpkeedagveas
pagasaavevetasqasdeegapasqasdeedapatdiyfptderswvys
plhysaqappasdgesdt