human ( Homo sapiens )
PI4KIIIalpha

Western result: +

control PI4KIIIalpha A1154b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: PI4KIIIalpha
CBI Antibody Core Code: A1154b
"Lot" Number: 1 Position: 20 (216-235)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
TISKKSGMSKKTNRGSQLHK

Antibody Name/Abbreviation: PI4KIIIalpha
CBI Antibody Core Code: A1154a
"Lot" Number: 1 Position: 100 (183-282)
% Identical Homology of Target to Mouse Protein: 25% (XP_129509.4| RIKEN cDNA 4933409L06)
Molecular Weight of fusion protein: 25.33 KDa

Target sequence used to make this antibody:
NQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSG
MSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPL
SAPELELDQAGENSVA^^^NWRS

Sequence Name: PI4KIIIalpha
Full name: phosphatidylinositol 4 kinase III alpha
UniGene Number: N.A.
Accession Number: AAA56839
Source organism: human ( Homo sapiens )
Full length size (aa): 854
NCBI information page:

Full length sequence:
MREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIW
IDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSLNIGGAKGSMNRHVAAIGP
RFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEK
RLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQA
TQGWINTYPLSSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLAT
EIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQWKDNVNLAW
SISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHT
IDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPD
AILFYIPQIVQALRYDKMGYVREYILWAASKSQLLAHQFIWNMKTNIYLD
EEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAII
KPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSA
AKAPYLAKFKVKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAI
FKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATAPGCGVIEC
IPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSL
LLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTD
EMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVTLMLDTGLPC
FRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQN
DIPY