human ( Homo sapiens )
MGC16664

Western result: +

GST GST-MGC16664 A1118a (fragment)

21.59 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MGC16664
CBI Antibody Core Code: A1118a
"Lot" Number: 1 Position: 68 (99-167)
% Identical Homology of Target to Mouse Protein: 89% (NP_808506.1| hypothetical protein A230106N23)
Molecular Weight of fusion protein: 21.59 KDa

Target sequence used to make this antibody:
SPYSSPFYIRTADMVPNGGGGERLSFAPTYYKEGGPPSLK
LAAPQSYPVTWPGSGREAFTNPRAISTDV

Western result: +

control MGC16664 A1118b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: MGC16664
CBI Antibody Core Code: A1118b
"Lot" Number: 1 Position: 20 (147-166)
% Identical Homology of Target to Mouse Protein: 90% (NP_808506.1| hypothetical protein A230106N23)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
VTWPGSGREAFTNPRAISTD

Sequence Name: MGC16664
Full name:
UniGene Number: N.A.
Accession Number: BC009382
Source organism: human ( Homo sapiens )
Full length size (aa): 167
NCBI information page:

Full length sequence:
MTAGTVVITGGILATVILLCIIAVLCYCRLQYYCCKKSGTEVADEEEERE
HDLPTHPRGPTCNACSSQALDGRGSLAPLTSEPCSQPCGVAASHCTTCSP
YSSPFYIRTADMVPNGGGGERLSFAPTYYKEGGPPSLKLAAPQSYPVTWP
GSGREAFTNPRAISTDV