human ( Homo sapiens )
G gamma12

Western result: +

control G gamma12 (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: G gamma12
CBI Antibody Core Code: A0397
"Lot" Number: 1 Position: 20(1-20)
% Identical Homology of Target to Mouse Protein: 100% (NP_079554.1| guanine nucleotide binding protein (G protein), gamma 12; EST AA536815; EST AI314170; EST AI115529)
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
MSSKTASTNSIAQARRTVQQ

Sequence Name: G gamma12
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: human ( Homo sapiens )
Full length size (aa): 72
NCBI information page:

Full length sequence:
MSSKTASTNSIAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSD
PLLIGIPTSENPFKDKKTCIIL