human ( Homo sapiens )
HCRTR2

Western result: +

GST GST-HCRTR2 A0991 (fragment)

32.5 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: HCRTR2
CBI Antibody Core Code: A0991
"Lot" Number: 1 Position: 50 (1-50)
% Identical Homology of Target to Mouse Protein: 92% (NP_945200.1| hypocretin (orexin) receptor 2)
Molecular Weight of fusion protein: 32.5 KDa

Target sequence used to make this antibody:
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFL
RYLWREYLHP

Antibody Name/Abbreviation: HCRTR2-pep
CBI Antibody Core Code: A0983
"Lot" Number: 1 Position: 20 (23-42)
% Identical Homology of Target to Mouse Protein: 100% (NP_945200.1| hypocretin (orexin) receptor 2)
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
ETQEPFLNPTDYDDEEFLRY

Sequence Name: HCRTR2
Full name: Human orexin receptor HCRTR2
UniGene Number: N.A.
Accession Number: N.A.
Source organism: human ( Homo sapiens )
Full length size (aa): 444
NCBI information page:

Full length sequence:
MSGTKLEDSPPCRNWSSASELNETQEPFLNPTDYDDEEFLRYLWREYLHP
KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLAD
VLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIAL
DRWYAICHPLMFKSTAKRARNSIVIIWIVSCIIMIPQAIVMECSTVFPGL
ANKTTLFTVCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKL
WCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARR
KTARMLMVVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSH
WLVYANSAANPIIYNFLSGKFREEFKAAFSCCCLGVHHRQEDRLTRGRTS
TESRKSLTTQISNFDNISKLSEQVVLTSISTLPAANGAGPLQNW